Differential expression in SAGE: accounting for normal between-library variation

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Differential expression in SAGE: accounting for normal between-library variation

MOTIVATION In contrasting levels of gene expression between groups of SAGE libraries, the libraries within each group are often combined and the counts for the tag of interest summed, and inference is made on the basis of these larger 'pseudolibraries'. While this captures the sampling variability inherent in the procedure, it fails to allow for normal variation in levels of the gene between in...

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Postnatal gene expression in the normal mouse cornea by SAGE.

PURPOSE To provide a detailed gene expression profile of the normal postnatal mouse cornea. METHODS Serial analysis of gene expression (SAGE) was performed on postnatal day (PN)9 and adult mouse (6 week) total corneas. The expression of selected genes was analyzed by in situ hybridization. RESULTS A total of 64,272 PN9 and 62,206 adult tags were sequenced. Mouse corneal transcriptomes are c...

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ژورنال

عنوان ژورنال: Bioinformatics

سال: 2003

ISSN: 1367-4803,1460-2059

DOI: 10.1093/bioinformatics/btg173